嗜酸性食管炎
高功率场
医学
骨膜炎
内科学
胃肠病学
病理
食管炎
嗜酸性粒细胞趋化因子
嗜酸性粒细胞
哮喘
疾病
免疫组织化学
细胞外基质
生物
回流
细胞生物学
作者
Amanda B. Muir,Steven J. Ackerman,Zhaoxing Pan,Alain Benitez,Cassandra Burger,Jonathan M. Spergel,Glenn T. Furuta,Joshua Rothman,Benjamin J. Wilkins,Michael Arnold,Lauren Dolinsky,Milica Grozdanovic,Calies Menard-Katcher
标识
DOI:10.1016/j.jaci.2022.03.022
摘要
Background
Esophageal remodeling is a factor in disease progression and symptom severity for patients with eosinophilic esophagitis (EoE). Remodeling can begin early in children, resulting in stricture and food impaction. Detection of esophageal remodeling often depends on endoscopy and is appreciated only in its later stages. Objective
We sought to determine whether luminal eosinophil-associated and remodeling proteins captured by the esophageal string test (EST) correlate with measures of esophageal remodeling and biomarkers of the epithelial–mesenchymal transition (EMT). Methods
Patients with EoE (7-18 years old) were enrolled from 2 pediatric hospitals. Participants performed the EST and underwent endoscopy. Histology, distensibility measured by endoluminal functional lumen imaging probe, and symptoms were assessed. Protein quantitation by ELISA was performed on mucosal biopsy and EST samples. Tissue sections were evaluated for EMT. Outcome measures were summarized, and Spearman ρ was used to assess bivariate correlations. Results
Forty patients (68% male) were enrolled (mean age, 12.5 years). Twenty-four (60%) had active disease (≥15 eosinophils per high-power field). EST-captured eotaxin-3, major basic protein 1, EDN, eosinophil peroxidase, and Charcot-Leyden crystal protein/galectin-10 showed significant correlations with peak eosinophils per high-power field (ρ 0.53-0.68, P < .001). Luminal proteins positively correlated with endoscopic features and markers of EMT, and negatively with esophageal distensibility. Periostin was captured by the EST and correlated with eosinophil density, basal zone hyperplasia, endoscopic appearance, and markers of EMT. Conclusion
Luminal markers of esophageal remodeling in addition to biomarkers of eosinophilic inflammation correlate with epithelial and functional remodeling in EoE.
科研通智能强力驱动
Strongly Powered by AbleSci AI