基因
水稻
超氧化物歧化酶
热休克蛋白
生物
转录组
丙二醛
转录因子
热冲击
交货地点
基因表达
植物
生物化学
酶
抗氧化剂
作者
Hang Zhou,Yingfeng Wang,Yijin Zhang,Yunhua Xiao,Xiong Liu,Huabing Deng,Xinya Lu,Wenbang Tang,Guilian Zhang
出处
期刊:Plants
[MDPI AG]
日期:2022-04-13
卷期号:11 (8): 1062-1062
被引量:15
标识
DOI:10.3390/plants11081062
摘要
To elucidate the mechanism underlying the response of rice to heat stress (HS), the transcriptome profile of panicles was comparatively analyzed between the heat-tolerant line 252 (HTL252) and heat-susceptible line 082 (HSL082), two rice recombinant inbred lines (RILs). Our differentially expressed gene (DEG) analysis revealed that the DEGs are mainly associated with protein binding, catalysis, stress response, and cellular process. The MapMan analysis demonstrated that the heat-responsive (HR) genes for heat shock proteins, transcription factors, development, and phytohormones are specifically induced in HTL252 under HS. Based on the DEG analysis, the key gene OsNCED1 (Os02g0704000), which was induced under HS, was selected for further functional validation. Moreover, 9-cis-epoxycarotenoid dioxygenase (NCED) is a key rate-limiting enzyme in the ABA biosynthetic pathway. Overexpression of OsNCED1 improved the HS tolerance of rice at the heading and flowering stage. OsNCED1-overexpression plants exhibited significant increases in pollen viability, seed setting rate, superoxide dismutase (SOD) and peroxidase (POD) activities, while significantly lower electrolyte leakage and malondialdehyde (MDA) content relative to the wild type (WT). These results suggested that OsNCED1 overexpression can improve the heat tolerance of rice by enhancing the antioxidant capacity. Overall, this study lays a foundation for revealing the molecular regulatory mechanism underlying the response of rice to prolonged HS.
科研通智能强力驱动
Strongly Powered by AbleSci AI