结核分枝杆菌
相互作用体
蛋白质组
生物
ATP合酶
药物发现
蛋白质组学
肺结核
生物化学
微生物学
细胞生物学
基因
医学
病理
作者
Luciana Dias Ghiraldi-Lopes,Paula Az Campanerut-Sá,Jean Eduardo Meneguello,Flávio Augusto Vicente Seixas,Mariana Aparecida Lopes-Ortiz,Regiane Bertin de Lima Scodro,Claudia T. A. Pires,Rosi Z da Silva,Vera Ld Siqueira,Celso Vataru Nakamura,Rosilene Fressatti Cardoso
出处
期刊:Future Microbiology
[Future Medicine]
日期:2017-08-01
卷期号:12 (10): 867-879
被引量:7
标识
DOI:10.2217/fmb-2017-0023
摘要
Aim: We investigated a proteome profile, protein–protein interaction and morphological changes of Mycobacterium tuberculosis after different times of eupomatenoid-5 (EUP-5) induction to evaluate the cellular response to the drug-induced damages. Methods: The bacillus was induced to sub-minimal inhibitory concentration of EUP-5 at 12 h, 24 h and 48 h. The proteins were separated by 2D gel electrophoresis, identified by LC/MS-MS. Scanning electron microscopy and Search Tool for the Retrieval of Interacting Genes/Proteins analyses were performed. Results: EUP-5 impacts mainly in M. tuberculosis proteins of intermediary metabolism and interactome suggests a multisite disturbance that contributes to bacilli death. Scanning electron microscopy revealed the loss of bacillary form. Conclusion: Some of the differentially expressed proteins have the potential to be drug targets such as citrate synthase (Rv0896), phosphoglycerate kinase (Rv1437), ketol-acid reductoisomerase (Rv3001c) and ATP synthase alpha chain (Rv1308).
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