生物
RNA聚合酶
分子生物学
核糖核酸
RNA依赖性RNA聚合酶
聚合酶
T7 RNA聚合酶
脊髓灰质炎病毒
大肠杆菌
RNA聚合酶Ⅰ
生物化学
抄写(语言学)
底漆(化妆品)
酶
噬菌体
病毒学
病毒
化学
基因
哲学
有机化学
语言学
作者
Stephen J. Plotch,Olga Palant,Y Gluzman
出处
期刊:Journal of Virology
[American Society for Microbiology]
日期:1989-01-01
卷期号:63 (1): 216-225
被引量:96
标识
DOI:10.1128/jvi.63.1.216-225.1989
摘要
A cDNA clone encoding the RNA polymerase of poliovirus has been expressed in Escherichia coli under the transcriptional control of a T7 bacteriophage promoter. The poliovirus enzyme was designed to contain only a single additional amino acid, the N-terminal methionine. The recombinant enzyme has been purified to near homogeneity, and polyclonal antibodies have been prepared against it. The enzyme exhibits poly(A)-dependent oligo(U)-primed poly(U) polymerase activity as well as RNA polymerase activity. In the presence of an oligo(U) primer, the enzyme catalyzes the synthesis of a full-length copy of either poliovirus or globin RNA templates. In the absence of added primer, RNA products up to twice the length of the template are synthesized. When incubated in the presence of a single nucleoside triphosphate, [alpha-32P]UTP, the enzyme catalyzes the incorporation of radioactive label into template RNA. These results are discussed in light of previously proposed models of poliovirus RNA synthesis in vitro.
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