清脆的
基因组编辑
Cas9
诱导多能干细胞
计算生物学
基因组
生物
基因
基因组工程
遗传学
胚胎干细胞
作者
Ciaran M. Lee,Hongxia Zhu,Timothy Davis,Harshahardhan Deshmukh,Gang Bao
出处
期刊:Methods in molecular biology
日期:2016-10-06
卷期号:: 3-21
被引量:6
标识
DOI:10.1007/978-1-4939-6472-7_1
摘要
The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.
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