牲畜
大肠杆菌
抗生素耐药性
抗生素
微生物学
生物
肠杆菌科
抗药性
兽医学
生物技术
医学
生态学
遗传学
基因
作者
Rosário Oliveira,Juliana Soares da Silva,Giarlã Cunha da Silva,Jéssica Nogueira Rosa,Denise Mara Soares Bazzolli,Hilário Cuquetto Mantovani
标识
DOI:10.1093/jambio/lxae058
摘要
Abstract Aim This study aimed to compare and characterize the resistance profile and the presence of ESBL-related genes in E. coli isolated from healthy finishing pigs fed with or without antibiotics in their diets. Methods and results A total of 27 ceftiofur-resistant E. coli isolates were obtained from 96 healthy pigs. The antibiotic resistance profile was tested, and all 27 isolates were classified as multidrug resistant (MDR). A high proportion of isolates were resistant to cephalosporins, ampicillin, ciprofloxacin, and tetracyclines. The ESBL production was observed in 85% of isolates by double-disc synergy test. The MDR-E. coli isolates harbored ESBL genes, such as blaTEM, blaCTX-M-1, blaCTX-M-2, and blaCTX-M-8,25. In addition, other ARGs were also detected, such as sul2, ant(3”)-I, tetA, and mcr-1. The mobilization of the blaCTX-M gene was confirmed for nine E. coli isolates by conjugation assays. The presence of blaCTX-M on mobile genetic elements in these isolates was demonstrated by Southern blot hybridization and the resistance to cephalosporins was confirmed in the transconjugants. Our results indicate the prevalence of CTX-M-producing E. coli strains harboring mobile genetic elements in the normal microbiota of healthy pigs. Conclusions These findings highlight the significance of ESBL genes as a global health concern in livestock and the potential spread of resistance to other members of the gastrointestinal tract microbiota.
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