穗
生物
苗木
基因
渗入
植物抗病性
遗传学
植物
作者
Ning Xiao,Yunyu Wu,Xiaoxiang Zhang,Zeyun Hao,Zichun Chen,Zefeng Yang,Yue Cai,Ruyi Wang,Ling Yu,Zhiping Wang,Yue Lu,Wei Shi,Cunhong Pan,Yuhong Li,Changhai Zhou,Jianju Liu,Niansheng Huang,Guangqing Liu,Hongjuan Ji,Shuhao Zhu,Shuai Fang,Yuese Ning,Aihong Li
出处
期刊:Molecular Plant
[Elsevier]
日期:2023-11-01
卷期号:16 (11): 1832-1846
被引量:6
标识
DOI:10.1016/j.molp.2023.10.001
摘要
Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most important diseases of rice. Utilization of blast-resistance genes is the most economical, effective, and environmentally friendly way to control the disease. However, genetic resources with broad-spectrum resistance (BSR) that is effective throughout the rice growth period are rare. In this work, using a genome-wide association study, we identify a new blast-resistance gene, Pijx, which encodes a typical CC-NBS-LRR protein. Pijx is derived from a wild rice species and confers BSR to M. oryzae at both the seedling and panicle stages. The functions of the resistant haplotypes of Pijx are confirmed by gene knockout and overexpression experiments. Mechanistically, the LRR domain in Pijx interacts with and promotes the degradation of the ATP synthase β subunit (ATPb) via the 26S proteasome pathway. ATPb acts as a negative regulator of Pijx-mediated panicle blast resistance, and interacts with OsRbohC to promote its degradation. Consistently, loss of ATPb function causes an increase in NAPDH content and ROS burst. Remarkably, when Pijx is introgressed into two japonica rice varieties, the introgression lines show BSR and increased yields that are approximately 51.59% and 79.31% higher compared with those of their parents in a natural blast disease nursery. In addition, we generate PPL
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