Nicotinamide boosts oocyte quantity and quality by promoting N4-acetylation modification in lupus mice

Patients with systemic lupus erythematosus (SLE) often have decreased fertility. Gene translation is crucial to oocyte meiosis and development. However, it remains unclear how SLE affects this process. Here, we used single-cell transcriptome and translatome sequencing to uncover a notable disruption in protein translation in oocytes from SLE mice, associated with the N 4 -acetylcytidine (ac 4 C) modification. Inhibition of ac 4 C levels in vitro substantially reduced oocyte translation efficiency. Notably, through trace-cell ac 4 C-RNA immunoprecipitation (acRIP) sequencing, we mapped the ac 4 C landscape in SLE mouse oocytes and found that deficient ac 4 C modification substantially impaired the translation of Zygote arrest 1 . Furthermore, we demonstrated that nicotinamide treatment notably and safely improved the quantity and quality of oocytes in SLE mice by enhancing ac 4 C modification levels. Our findings highlight the essential role of N - acetyltransferase 10 (NAT10)-mediated ac 4 C modification in abnormal oocyte development in SLE and suggest that nicotinamide holds promise for improving fertility in patients with SLE.