生物
RNA甲基化
转录组
N6-甲基腺苷
核糖核酸
基因表达谱
细胞生物学
甲基化
信使核糖核酸
基因表达
计算生物学
分子生物学
遗传学
作者
Matthew Tegowski,Mathieu N. Flamand,Kate D. Meyer
出处
期刊:Molecular Cell
[Elsevier]
日期:2022-02-01
卷期号:82 (4): 868-878.e10
被引量:8
标识
DOI:10.1016/j.molcel.2021.12.038
摘要
Summary
N6-methyladenosine (m6A) is an abundant RNA modification that plays critical roles in RNA regulation and cellular function. Global m6A profiling has revealed important aspects of m6A distribution and function, but to date such studies have been restricted to large populations of cells. Here, we develop a method to identify m6A sites transcriptome-wide in single cells. We uncover surprising heterogeneity in the presence and abundance of m6A sites across individual cells and identify differentially methylated mRNAs across the cell cycle. Additionally, we show that cellular subpopulations can be distinguished based on their RNA methylation signatures, independent from gene expression. These studies reveal fundamental features of m6A that have been missed by m6A profiling of bulk cells and suggest the presence of cell-intrinsic mechanisms for m6A deposition.
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