弓形虫
基因型
膀胱癌
血清流行率
癌症
宫颈癌
内科学
生物
套式聚合酶链反应
医学
免疫学
抗体
胃肠病学
聚合酶链反应
血清学
基因
遗传学
作者
Wei Cong,Guohua Li,Qing-Feng Meng,Dong Wang,Siyuan Qin,Fu-Kai Zhang,Xiangyan Zhang,Xiangyang Wang,Qian Ai-dong,Xing‐Quan Zhu
出处
期刊:Cancer Letters
[Elsevier]
日期:2015-04-01
卷期号:359 (2): 307-313
被引量:102
标识
DOI:10.1016/j.canlet.2015.01.036
摘要
Prevalence of human infection with Toxoplasma gondii has been increasing in China due to the increasing number of cats. However, little is known of the epidemiology of T. gondii infection in different cancer patient groups. Thus, a case–control study of 900 cancer patients and 900 controls was conducted to detect anti-T. gondii antibodies by ELISA in China. Genomic DNA was extracted from the diseased tissues of 510 patients and the T. gondii B1 gene was amplified using a semi-nested PCR. DNA samples giving positive B1 amplification were then genetically characterized using multi-locus PCR-RFLP. The prevalence of anti-T. gondii IgG in cancer patients (35.56%) was significantly higher than that in controls (17.44%). The highest T. gondii seroprevalence was detected in lung cancer patients (60.94%), followed by cervical cancer patients (50%), brain cancer patients (42.31%) and endometrial cancer patients (41.67%). Exposure with soil and consumption of raw/undercooked meat were significantly associated with T. gondii infection in cancer patients. Three T. gondii genotypes (ToxoDB#9, ToxoDB#10 and Type I variant) were identified. In conclusion, T. gondii infection is a severe problem in cancer patients and it is imperative that improved integrated measures should be conducted to prevent and control T. gondii infection in cancer patients.
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