冬虫夏草
生物
蛋白质细菌
当归
山茶
植物
真菌多样性
微生物种群生物学
放线菌门
生态学
细菌
16S核糖体RNA
替代医学
中医药
病理
医学
遗传学
作者
Jing Yang,Xinxin Tong,Chengyan He,Jing Bai,Fang Wang,Jinlin Guo
标识
DOI:10.19540/j.cnki.cjcmm.20210226.103
摘要
To obtain the difference of the fungal and bacterial community diversity between wild Cordyceps sinensis, artificial C. sinensis and their habitat soil, Illmina Hiseq high-throughput sequencing technology was applied. The results show that Proteobacteria was the dominant bacterial phylum in C. sinensis, Actinobacteria was the dominant bacterial phylum in soil microhabitat, Ophiocordyceps sinensis was the predominant dominant fungus of C. sinensis. The α diversity analysis showed that the fungal diversity of stroma was lower than other parts, and the fungal diversity of wild C. sinensis was lower than that of artificial C. sinensis. The β diversity analysis showed that the fungal and bacterial community diversity of soil microhabitat samples was significantly different from that of C. sinensis. The fungal community diversity was less different between wild and artificial C. sinensis, especially in sclerotia. LEfSe analysis showed a lot of species diversity between wild and artificial C. sinensis. Those different species between wild C. sinensis, artificial C. sinensis and their habitat soil provide ideas for further research on breed and components of C. sinensis.
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