Qualitative Analysis and Determination of Total Phenolic And Total Flavonoid Content Of Needles Of Pinus roxburghii From Srinagar Garhwal

Pinus roxburghii is a gymnosperm tree species, which is abundantly found in the north western Himalayan region and possess medicinal properties. In this study, extracts of Pinus roxburghii needles were made in different solvents which were used for phytochemical screening. The extracts were used to carry out the study of the total phenolic content and the total flavonoid content using Folin-Ciocalteu method and aluminium chloride method respectively. Methanolic extract of Pinus roxburghii needles showed the maximum amount of total phenolic content (TPC) and total flavonoid content (TFC) that were 306.7053 ± 0.88711 GAE/g and 188.1283 ± 0.09029 RE/g respectively. It was concluded that the presence of secondary metabolites can be further investigated for medicinal purposes.