化学
替考拉宁
催化作用
水解
细菌
克
过氧化氢酶
微生物学
生物化学
酶
万古霉素
金黄色葡萄球菌
遗传学
生物
作者
Yue Wu,Shan Jiang,Zhifeng Fu
出处
期刊:Talanta
[Elsevier]
日期:2020-05-01
卷期号:211: 120728-120728
被引量:14
标识
DOI:10.1016/j.talanta.2020.120728
摘要
Based on the specific affinity behavior of teicoplanin towards Gram-positive (G+) bacteria, teicoplanin-coated magnetic particles were employed to separate G+ bacteria. Moreover, intracellular catalase released by catalase-positive bacteria led to catalyzed hydrolysis of H2O2 and inhibition of chemiluminescent signal of luminol-H2O2 system. By combining the separation capability of teicoplanin-coated magnetic particles and the inhibition of luminol chemiluminescence by catalase, a facile method was proposed for quantifying catalase-positive G+ bacteria. In this proof-of-principle work, Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus were adopted as model bacteria to evaluate its quantification performance. The three bacteria were quantified within the dynamic ranges of 5.0 × 102-5.0 × 107 CFU mL-1, 4.0 × 102-4.0 × 107 CFU mL-1 and 2.0 × 102-2.0 × 107 CFU mL-1, with detection limits of 156 CFU mL-1, 86 CFU mL-1 and 44 CFU mL-1, respectively. Gram-negative bacteria and catalase-negative G+ bacteria both displayed minor interference. The results for quantifying bacteria in milk, human urine and physiological saline samples demonstrated its reliability for real application. It provided a promising technique tool for food safety, medical diagnosis as well as drug quality control.
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