适体
循环肿瘤细胞
检出限
电极
转移
线性范围
纳米技术
电化学
癌症转移
化学
材料科学
组合化学
癌症研究
癌症
分子生物学
色谱法
生物
物理化学
遗传学
作者
Juan Cai,Huawei Shen,Yu Wang,Yang Peng,Sitian Tang,Yalin Zhu,Qian Liu,Baiying Li,Guoming Xie,Wenli Feng
标识
DOI:10.1016/j.bios.2020.112893
摘要
Accurate detection of circulating tumor cells (CTCs) has a pivotal role in the metastasis monitoring and prognosis of tumor. In this work, an ultrasensitive electrochemical cytosensor was developed based on excellent electrocatalytic materials and a dual recognition strategy. Herein, novel branched PtAuRh trimetallic nanospheres (b-PtAuRh TNS) were synthesized for the first time by a facile one-pot method, which had a huge specific surface area and outstanding catalytic activity. B-PtAuRh TNS linked with aptamers targeting mucin1 (MUC1) were served as signal tags to amplify the signal. As electrode modified material, the nanocomposites of Cabot carbon black (BP2000) and AuNPs were used to improve the electron transfer efficiency of electrode. In addition to using b-PtAuRh TNS labeled anti-MUC1 aptamers as signal probes, anti-EpCAM antibodies were worked as capture probes to achieve dual recognition of target cells. In other words, only cells expressing both MUC1 and EpCAM could produce electrochemical signal. The constructed cytosensor presented a wide linear range (5 - 1 × 106 cells mL−1) and a low detection limit (1 cell mL−1). It was worth noting that the proposed cytosensor could detect CTCs in clinical blood samples. To sum up, the developed cytosensor might become a promising detection platform for cancer diagnosis and tumor metastasis.
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