蛋白质设计
计算机科学
蛋白质-蛋白质相互作用
蛋白质功能
计算生物学
支架蛋白
主题(音乐)
功能(生物学)
蛋白质结构
生物
细胞生物学
信号转导
生物化学
物理
声学
基因
作者
Daniel‐Adriano Silva,Bruno E. Correia,Erik Procko
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 285-304
被引量:32
标识
DOI:10.1007/978-1-4939-3569-7_17
摘要
Protein–protein interfaces regulate many critical processes for cellular function. The ability to accurately control and regulate these molecular interactions is of major interest for biomedical and synthetic biology applications, as well as to address fundamental biological questions. In recent years, computational protein design has emerged as a tool for designing novel protein–protein interactions with functional relevance. Although attractive, these computational tools carry a steep learning curve. In order to make some of these methods more accessible, we present detailed descriptions and examples of ROSETTA computational protocols for the design of functional protein binders using seeded protein interface design. In these protocols, a motif of known structure that interacts with the target site is grafted into a scaffold protein, followed by design of the surrounding interaction surface.
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