衍生化
化学
结构异构体
双键
磷脂酰胆碱
质谱法
职位(财务)
立体化学
色谱法
磷脂
有机化学
生物化学
膜
财务
经济
作者
Qiongqiong Wan,Yanan Xiao,Guifang Feng,Xin Dong,Wenjing Nie,Ming Gao,Qingtao Meng,Suming Chen
标识
DOI:10.1016/j.cclet.2023.108775
摘要
There is a close relationship between the biological functions of lipids and their structures, and various isomers greatly increases the complexity of lipid structures. The C=C bond location and sn-position are two of the essential attributes that determine the structures of unsaturated lipids. However, simultaneous identification of both attributes remains challenging. Here, we develop a visible-light-activated aziridination reaction system, which enables the dual-resolving of the C=C bond location and sn-position isomerism of in lipids when combines with liquid chromatography-mass spectrometry (LC-MS). Based on the derivatization of C=C bonds with PhI=NTs, their location in lipids could be easily identified by tandem MS. Especially, the sn-position isomers of unsaturated phosphatidylcholine (PC) can be separated and quantified by LC-MS after the derivatization. By using the proposed method, the significant changes of the sn-position isomers ratios of PC in mouse brain ischemia were revealed. This study offers a powerful tool for deep lipid structural biology.
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