Wound healing involves four phases, namely hemostasis, inflammation, proliferation, and remodeling. The four meticulously and carefully programmed stages make up the normal biological process of wound healing in the human body. These phases must occur in the proper order and time for a wound to heal rapidly. Praseodymium oxide (PrO2) was synthesized using combustion method. The fibers were electro-spun using a polymer solution containing Poly (vinyl alcohol) (PVA) and sodium alginate (SA) along with PrO2. The morphology and the crystalline structure were characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), FT-IR and scanning electron microscopy (SEM). PrO2 is incorporated into electro-spun fibers to enhance the wound healing process. The antimicrobial activity of PrO2 was tested by growth curve analysis, biofilm, colony count and well diffusion methods. Swelling, degradation, porosity and water vapor transmission rate were also performed. PrO2 incorporated fiber have shown a faster recovery rate and faster wound closure in scratch assay and MTT assay. PVA/SA/PrO2 fiber shows an enhanced wound healing in the in-vivo model.