生物
分泌物
电池类型
细胞
杯状细胞
细胞分化
干细胞
肠上皮
恶性肿瘤
大肠
细胞生物学
上皮
癌症研究
内分泌学
生物化学
遗传学
基因
作者
Isabel Puig,Irene Chicote,Héctor G Pálmer
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 227-233
标识
DOI:10.1007/978-1-0716-3076-1_17
摘要
The intestinal epithelium is a rapid self-renewing tissue. Stem cells at the bottom of the crypts first give rise to a proliferative progeny that finally differentiates to a variety of cell types. These terminally differentiated intestinal cells are mostly present in the villi of the intestinal wall and serve as functional units to sustain the main purpose of the organ: food absorption. But for a balance homeostasis, the intestine is composed not only by absorptive enterocytes but also by other cell types such as goblet cells that secrete mucus to lubricate the intestinal lumen, Paneth cells that secrete antimicrobial peptides to control microbiome, and others. Many relevant conditions affecting the intestine including chronic inflammation, Crohn's disease, or cancer can alter the composition of these different functional cell types. As a consequence, they can lose their specialized activity as functional units and further contribute to disease progression and malignancy. Measuring the amount of these different cell populations in the intestine is essential to understand the bases of these diseases and their specific contribution to their malignancy. Interestingly, patient-derived xenograft (PDX) models faithfully recapitulate patients' tumors including the proportion of the different cell lineages present in the original tumor. Here we expose some protocols for evaluating the differentiation of intestinal cells in colorectal tumors.
科研通智能强力驱动
Strongly Powered by AbleSci AI