透皮
渗透
树枝状大分子
化学
体内
渗透(战争)
真皮
角质层
生物物理学
生物医学工程
色谱法
药理学
高分子化学
医学
生物化学
膜
解剖
病理
生物技术
工程类
生物
运筹学
作者
Katarzyna Borowska,Stanislaw Wołowiec,Andrzej Rubaj,Kazimierz Głowniak,Elwira Sieniawska,Sebastian Radej
标识
DOI:10.1016/j.ijpharm.2012.01.041
摘要
In the present study we have assessed the ability of (PAMAM) dendrimers G3 and G4 to facilitate transdermal delivery of 8-methoxypsoralen (8-MOP) in vivo. In vitro study using Franz diffusion cell revealed an enhanced transdermal flux for 8-MOP in complex with G3 and G4 dendrimer in relation to standard 8-MOP solution. In present study in vivo skin permeation potential of 8-MOP complex with G3 and G4 PAMAM dendrimer was assessed using confocal laser scanning microscopy (CLSM), which revealed an enhanced permeation of the 8-MOP to the deeper layers of the skin and significantly higher concentration in comparison with standard 8-MOP solution. Skin tissue 8-MOP concentration, evaluated by HPLC indicates that G3 and G4 PAMAM application significantly increase 8-MOP skin deposition in comparison with standard 8-MOP solutions after 1 and 2 h. G4 appeared to be a more effective 8-MOP penetration enhancer than G3 PAMAM. Our results suggest the feasibility of G3 and G4 PAMAM dendrimers for transdermal delivery of 8-MOP resulting in better skin permeation and higher concentration of 8-MOP in epidermis and dermis of the drug that could help to improve effectiveness and safety of PUVA therapy.
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