Protein-Restricted Diet Regulates Lipid and Energy Metabolism in Skeletal Muscle of Growing Pigs

The aim of this work was to study the lipid metabolism and energy status of skeletal muscle of pigs as affected by dietary protein restriction. Eighteen growing pigs were distributed into three treatments, and each group was fed one of three levels (20, 17, and 14%) of crude protein (CP) diets. Our results showed that pigs fed the 20% CP had greater (P < 0.05) gain:feed and muscle weight than those fed the 14% CP, but no differences between the 20 and 17% CP treatments. Additionally, protein restriction tended to increase (P = 0.07) the content of intramuscular fat (IMF) and up-regulated (P < 0.05) expression of lipogenic-related genes. Energy status was changed and, concomitantly, AMP-activated protein kinase α pathway was inhibited by reducing the dietary protein level. These results indicate protein restriction could be useful to improve IMF content of pigs through regulating lipid metabolism and associated energy utilization in muscle.