化学
检出限
石墨烯
荧光
生物传感器
堆积
量子点
光化学
猝灭(荧光)
分析化学(期刊)
纳米技术
色谱法
有机化学
量子力学
物理
生物化学
材料科学
作者
Xiaotong Liu,Weidan Na,Qing Liu,Xingguang Su
标识
DOI:10.1016/j.aca.2018.04.038
摘要
In this work, we presented a novel label-free biosensor for rapid detection of bleomycinsulphate (BLM). The biosensor was based on the fluorescent "turn off-on" of nitrogen-doped graphene quantum dots (N-GQDs), which was prepared in a green way from citric acid and ammonia. The richness of carboxyl groups on the N-GQDs enabled strong adsorption of ssDNA to the surface of N-GQDs through π-π stacking interactions, resulting in the effective fluorescence quenching of N-GQDs system. The ssDNA underwent an irreversible cleavage event via the oxidative effect of BLM with Fe(II) as a cofactor, thus a turn-on fluorescence signal was observed. Thereby, the concentration of BLM can be quantitatively determined in a broad range from 0.34 nmol/L to 1300 nmol/L with a detection limit of 0.34 nmol/L. The presented method was applied to the determination of BLM in human serum samples with satisfactory results.
科研通智能强力驱动
Strongly Powered by AbleSci AI