Development of a multiplex PCR procedure for detection of Yersinia genus with identification of pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica)

To identify the bacteria of the Yersinia genus and pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) in a single reaction, a multiplex PCR technique, which uses genes of nonspecific porins (OmpF-like proteins), has been developed; it was optimized by five PCR buffer compounds and the temperature of primer annealing. Detection efficiency of genus-and species-specific primers was determined. The multiplex PCR provides an improved rapid technique for detecting the Yersinia genus and identifying pathogenic species.