Chitosan/pullulan based films incorporated with clove essential oil loaded chitosan-ZnO hybrid nanoparticles for active food packaging

Herein, we developed clove essential oil (CEO) loaded Chitosan-ZnO hybrid nanoparticles (CS-ZnO@CEO (CZC NPs)) integrated chitosan/pullulan (CS/PL) nanocomposite films. SEM images revealed a homogenous distribution of CZC NPs with minimum aggregation in nanocomposite films. The incorporation of CZC NPs led to enhanced tensile strength (~39.82%), film hydrophobicity (~35.36%), UV light blocking ability, water vapor barrier (~84.64%), and oxygen barrier (~57.66%) compared to the bare CS/PL film and overall migration limit of CPCZC films were found below the permitted limit of 1000 μg/dm2. Besides, incorporation of CZC NPs into the CS/PL films enhanced antioxidant activity and showed strong antibacterial activity against P. aeruginosa, S. aureus, and E. coli. Also, the CPCZC films displayed potential to extend the shelf-life of chicken meat by up to 5 days when stored at 8 ± 2 °C. These results suggest that the prepared CPCZC films acquire the ideal prerequisites for potential active packaging materials.