癌变
免疫系统
转移
癌症研究
医学
癌症
肺癌
免疫学
病理
内科学
作者
Courtney Smith,Mee Young Chang,Katherine H. Parker,Daniel W. Beury,James B. DuHadaway,Hollie E. Flick,Janette Boulden,Erika Sutanto‐Ward,Aléjandro Peralta Soler,Lisa D. Laury‐Kleintop,Laura Mandik-Nayak,Richard Metz,Suzanne Ostrand‐Rosenberg,George C. Prendergast,Alexander J. Muller
出处
期刊:Cancer Discovery
[American Association for Cancer Research]
日期:2012-08-01
卷期号:2 (8): 722-735
被引量:271
标识
DOI:10.1158/2159-8290.cd-12-0014
摘要
Indoleamine 2,3-dioxygenase (IDO) enzyme inhibitors have entered clinical trials for cancer treatment based on preclinical studies, indicating that they can defeat immune escape and broadly enhance other therapeutic modalities. However, clear genetic evidence of the impact of IDO on tumorigenesis in physiologic models of primary or metastatic disease is lacking. Investigating the impact of Ido1 gene disruption in mouse models of oncogenic KRAS-induced lung carcinoma and breast carcinoma-derived pulmonary metastasis, we have found that IDO deficiency resulted in reduced lung tumor burden and improved survival in both models. Micro-computed tomographic (CT) imaging further revealed that the density of the underlying pulmonary blood vessels was significantly reduced in Ido1-nullizygous mice. During lung tumor and metastasis outgrowth, interleukin (IL)-6 induction was greatly attenuated in conjunction with the loss of IDO. Biologically, this resulted in a consequential impairment of protumorigenic myeloid-derived suppressor cells (MDSC), as restoration of IL-6 recovered both MDSC suppressor function and metastasis susceptibility in Ido1-nullizygous mice. Together, our findings define IDO as a prototypical integrative modifier that bridges inflammation, vascularization, and immune escape to license primary and metastatic tumor outgrowth.This study provides preclinical, genetic proof-of-concept that the immunoregulatory enzyme IDO contributes to autochthonous carcinoma progression and to the creation of a metastatic niche. IDO deficiency in vivo negatively impacted both vascularization and IL-6–dependent, MDSC-driven immune escape, establishing IDO as an overarching factor directing the establishment of a protumorigenic environment.
科研通智能强力驱动
Strongly Powered by AbleSci AI