生物
粳稻
水稻
野生稻
氨基酸
基因
单倍型
稻属
转基因水稻
穗
基因型
植物
遗传学
转基因
转基因作物
作者
Yi Shi,Yuanyuan Zhang,Yangyang Sun,Zhihui Xie,Yan Luo,Qiyuan Long,Jianghua Feng,Xueqing Liu,Wang Bi,Dujun He,Junxia Ren,Peizhen Guo,Junwei Xing,Ling He,Alisdair R. Fernie,Wei Chen,Xianqing Liu,Yuqin Luo,Cheng Jin,Jie Luo
标识
DOI:10.1016/j.molp.2022.12.013
摘要
Grain essential amino acid (EAA) levels contribute to rice nutritional quality. However, the molecular mechanisms underlying EAA accumulation and natural variation in rice grains remain unclear. Here we report the identification of a previously unrecognized auxin influx carrier subfamily gene, OsAUX5, which encodes an amino acid transporter that functions in uptake of multiple amino acids. We identified an elite haplotype of Pro::OsAUX5Hap2 that enhances grain EAA accumulation without an apparent negative effect on agronomic traits. Natural variations of OsAUX5 occur in the cis elements of its promoter, which are differentially activated because of the different binding affinity between OsWRKY78 and the W-box, contributing to grain EAA variation among rice varieties. The two distinct haplotypes were shown to have originated from different Oryza rufipogon progenitors, which contributed to the divergence between japonica and indica. Introduction of the indica-type Pro::OsAUX5Hap2 genotype into japonica could significantly increase EAA levels, indicating that indica-type Pro::OsAUX5Hap2 can be utilized to increase grain EAAs of japonica varieties. Collectively, our study uncovers an WRKY78-OsAUX5-based regulatory mechanism controlling grain EAA accumulation and provides a potential target for breeding EAA-rich rice.
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