生物
前列腺癌
分子生物学
小RNA
报告基因
免疫印迹
癌症
癌症研究
基因
核糖核酸
基因表达
遗传学
作者
Hanlin Li,Yanjie Yang,Jianpeng Yu,Boya Zhang,Xuanrong Chen,Shimiao Zhu,Yuanjie Niu,Zhiqun Shang
出处
期刊:Epigenomics
[Future Medicine]
日期:2022-07-01
卷期号:14 (13): 823-836
被引量:2
标识
DOI:10.2217/epi-2022-0111
摘要
Aims: We aimed to determine whether intronic circRNA acts as a molecular sponge in castration-resistant prostate cancer (CRPC). Materials & methods: A gene chip technique was used to conduct sequencing. A qPCR experiment was performed to verify the result. Radioimmunoprecipitation, RNA pull-down and dual-luciferase reporter assays were performed to particularly expound its function. Verification of downstream effects was carried out through qPCR and western blot, and a xenograft assay was performed in vivo for verification. Results: Intronic circRNA hsa_circ_0092339 in the nucleus was highly expressed in CRPC cell lines. hsa_circ_0092339 did not regulate the expression of its parental gene. hsa_circ_0092339 functions like a molecular sponge, preventing degradation of C-MYC mRNA by absorbing hsa-mir-940. Conclusion: hsa_circ_0092339 plays a critical role in CRPC through targeting C-MYC indirectly by absorbing hsa-mir-940.Our research breaks the mold by investigating a novel function of RNA and a novel regulatory mechanism. Our research provides a new therapeutic target for prostate cancer treatment and broadens the understanding of prostate cancer.
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