小胶质细胞
转录组
表观遗传学
生物
染色质
基因表达
计算生物学
核糖核酸
基因
神经科学
适应(眼睛)
细胞生物学
遗传学
炎症
免疫学
作者
Rebekka Scholz,Desirée Brösamle,Xidi Yuan,Jonas J. Neher,Marc Beyer
出处
期刊:Methods in molecular biology
日期:2023-08-29
卷期号:: 543-571
被引量:1
标识
DOI:10.1007/978-1-0716-3437-0_35
摘要
The advance of single-cell RNA-sequencing technologies in the past years has enabled unprecedented insights into the complexity and heterogeneity of microglial cell states in the homeostatic and diseased brain. This includes rather complex proteomic, metabolomic, morphological, transcriptomic, and epigenetic adaptations to external stimuli and challenges resulting in a novel concept of core microglia properties and functions. To uncover the regulatory programs facilitating the rapid transcriptomic adaptation in response to changes in the local microenvironment, the accessibility of gene bodies and gene regulatory elements can be assessed. Here, we describe the application of a previously published method for simultaneous high-throughput ATAC and RNA expression with sequencing (SHARE-seq) on microglia nuclei isolated from frozen mouse brain tissue.
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