运行x2
碱性磷酸酶
生物相容性
茜素红
染色
牙周膜干细胞
化学
再生(生物学)
骨愈合
骨钙素
核化学
材料科学
生物医学工程
解剖
病理
细胞生物学
生物
生物化学
医学
酶
有机化学
作者
Y Li,S. Guo,Julian Choi,Ju‐Lan Zeng,Jingwen Zhang,Fuxiang Zhao,Chun‐dong Liu,Xiaoyun Shen,Yuan‐Ming Geng
摘要
Abstract This study explores the use of in situ cross‐linked hyaluronic acid methacryloyl (HAMA) and hydroxyapatite particles (HAP) for bone defect repair. Human periodontal ligament stem cells (PDLSCs) were isolated and co‐cultured with the HAMA‐HAP composite. Osteogenic differentiation was evaluated using Alizarin Red staining, alkaline phosphatase activity quantification, and polymerase chain reaction (PCR). A cranial defect was induced in Sprague–Dawley rats. This defect was then filled with the HAMA‐HAP composite and cross‐linked using UV light exposure. Bone formation was assessed through radiographic and histological analyses. The HAMA‐HAP composite was found to promote cell viability similarly to pure HAP. It also enhanced gene expression of ALP, OPN, and Runx2, and increased ALP activity and mineralized nodule formation in vitro. Micro‐CT scans showed defect restoration in the HAMA‐HAP and HAP groups compared to the control group. The HAMA‐HAP group exhibited higher Tb.N, Tb.Sp, Tb.Th, and BV/TV. Masson staining showed the HAMA‐HAP composite restored the defect site, with new bone formation thicker than in the HAP group. The HAMA‐HAP composite showed excellent biocompatibility and promoted osteogenic differentiation of PDLSCs. It effectively repaired cranial defects, indicating its potential for clinical use in bone defect repair.
科研通智能强力驱动
Strongly Powered by AbleSci AI