生物加工
自愈水凝胶
3D生物打印
材料科学
生物医学工程
神经组织工程
透明质酸
组织工程
再生医学
聚合物
细胞外基质
活力测定
光致聚合物
化学
细胞
高分子化学
解剖
复合材料
生物化学
生物
单体
医学
作者
Alexander P. Haring,Earl A. Thompson,Yuxin Tong,Sahil Laheri,Ellen Cesewski,Harald Sontheimer,Blake N. Johnson
出处
期刊:Biofabrication
[IOP Publishing]
日期:2019-02-25
卷期号:11 (2): 025009-025009
被引量:70
标识
DOI:10.1088/1758-5090/ab02c9
摘要
A bio-inspired hydrogel for 3D bioprinting of soft free-standing neural tissues is presented. The novel filler-free bioinks were designed by combining natural polymers for extracellular matrix biomimicry with synthetic polymers to endow desirable rheological properties for 3D bioprinting. Crosslinking of thiolated Pluronic F-127 with dopamine-conjugated (DC) gelatin and DC hyaluronic acid through a thiol-catechol reaction resulted in thermally gelling bioinks with Herschel-Bulkley fluid rheological behavior. Microextrusion 3D bioprinting was used to fabricate free-standing cell-laden tissue constructs. The bioinks exhibited flattened parabolic velocity profiles with tunable low shear regions. Two pathways were investigated for curing the bioink: chelation and photocuring. The storage modulus of the cured bioinks ranged from 6.7 to 11.7 kPa. The iron (III) chelation chemistry produced crosslinked neural tissues of relatively lower storage modulus than the photocuring approach. In vitro cell viability studies using the 3D bioprinted neural tissues showed that the cured bioink was biocompatible based on minimal cytotoxic response observed over seven days in culture relative to control studies using alginate hydrogels. Rodent Schwann cell-, rodent neuronal cell-, and human glioma cell-laden tissue constructs were printed and cultured over seven days and exhibited comparable viability relative to alginate bioink controls. The ability to fabricate soft, free-standing 3D neural tissues with low modulus has implications in the biofabrication of microphysiological neural systems for disease modeling as well as neural tissues and innervated tissues for regenerative medicine.
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