化学
质谱法
质谱成像
解吸
基质(化学分析)
基质辅助激光解吸/电离
马尔迪成像
质谱
分析化学(期刊)
电离
色谱法
离子
有机化学
吸附
作者
Egoitz Astigarraga,Gabriel Barreda‐Gómez,Laura Lombardero,Olatz Fresnedo,Fernando Castaño,M.T. Giralt,Begoña Ochoa,Rafael Rodríguez‐Puertas,José A. Fernández
摘要
2-Mercaptobenzothiazole (MBT) is employed for the first time as a matrix for the analysis of lipids from tissue extracts using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. We demonstrate that the performance of MBT is superior to that of the matrixes commonly employed for lipids, due to its low vapor pressure, its low acidity, and the formation of small crystals, although because of the strong background at low m/z, it precludes detection of species below approximately 500 Da. This inconvenience can be partly overcome with the formation of Cs adducts. Using a polymer-based dual calibration, a mass accuracy of approximately 10 ppm in lipid extracts and of approximately 80 ppm in tissues is achieved. We present spectra from liver and brain lipid extracts where a large amount of lipid species is identified, in both positive and negative ion modes, with high reproducibility. In addition, the above-mentioned special properties of MBT allow its employment for imaging mass spectrometry. In the present work, images of brain and liver tissues showing different lipid species are presented, demonstrating the advantages of the employment of MBT.
科研通智能强力驱动
Strongly Powered by AbleSci AI