生物
滋养层
胚胎干细胞
转录组
细胞生物学
体外
细胞分化
干细胞
诱导多能干细胞
分子生物学
遗传学
基因
基因表达
胎儿
怀孕
胎盘
作者
Zhuoran Yu,Sihan Zhou,Minli Liu,Lijun Chen,Xue Zhang,Xu Yang,Hongdi Cui,Chaoqian Jiang,Yanjiao Lv,Tingsheng Yan,Qingran Kong,Zhonghua Liu
出处
期刊:Stem Cells
[Wiley]
日期:2022-05-18
卷期号:40 (8): 751-762
被引量:3
标识
DOI:10.1093/stmcls/sxac034
摘要
Pluripotent stem cells (PSCs) have unlimited self-renewal and multifunctional development potential in vitro. Porcine PSCs are highly desirable due to the conserved characteristics between pigs and humans. Extended PSCs (EPSCs) are additionally capable of differentiating into embryonic (Em) and extraembryonic (E×Em) parts. Here, we employed the LCDM culture system (consisting of human LIF, CHIR99021, (S)-(+)-dimethindene maleate, and minocycline hydrochloride), which can establish EPSCs from humans and mice, to derive and maintain stable porcine PSCs (pLCDM) from in vivo blastocysts. Transcriptome analysis revealed the unique molecular characteristics of pLCDMs compared with early-stage embryos. Meanwhile, the parallels and differences in the transcriptome features among pLCDMs, human EPSCs, and mouse EPSCs were carefully analyzed and evaluated. Most noteworthy, the trophoblast lineage differentiation tendency of pLCDMs was clarified by inducing trophoblast-like cells and trophoblast stem cells (TSCs) in vitro. Further research found that 2 of the small molecules in LCDM culture system, (S)-(+)-dimethindene maleate (DiM) and minocycline hydrochloride (MiH), probably play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
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