间充质干细胞
细胞生物学
再生(生物学)
线粒体
细胞外基质
化学
再生医学
干细胞
生物
作者
Jun Bai,Bin Yu,Chaochao Li,Haofeng Cheng,Yanjun Guan,Zhiqi Ren,Tieyuan Zhang,Xiangyu Song,Zhibo Jia,Tao Su,Benzhang Tao,Haihao Gao,Boyao Yang,Lijing Liang,Xuejian Xiong,Xingyu Zhou,Lan Yin,Jiang Peng,Aijia Shang,Yu Wang
标识
DOI:10.1002/adhm.202302128
摘要
Peripheral nerve injuries (PNI) can lead to mitochondrial dysfunction and energy depletion within the affected microenvironment. The objective is to investigate the potential of transplanting mitochondria to reshape the neural regeneration microenvironment. High-purity functional mitochondria with an intact structure are extracted from human umbilical cord-derived mesenchymal stem cells (hUCMSCs) using the Dounce homogenization combined with ultracentrifugation. Results show that when hUCMSC-derived mitochondria (hUCMSC-Mitos) are cocultured with Schwann cells (SCs), they promote the proliferation, migration, and respiratory capacity of SCs. Acellular nerve allografts (ANAs) have shown promise in nerve regeneration, however, their therapeutic effect is not satisfactory enough. The incorporation of hUCMSC-Mitos within ANAs has the potential to remodel the regenerative microenvironment. This approach demonstrates satisfactory outcomes in terms of tissue regeneration and functional recovery. Particularly, the use of metabolomics and bioenergetic profiling is used for the first time to analyze the energy metabolism microenvironment after PNI. This remodeling occurs through the enhancement of the tricarboxylic acid cycle and the regulation of associated metabolites, resulting in increased energy synthesis. Overall, the hUCMSC-Mito-loaded ANAs exhibit high functionality to promote nerve regeneration, providing a novel regenerative strategy based on improving energy metabolism for neural repair.
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