| 标题 |
 Protein Production using the Baculovirus Expression System 利用杆状病毒表达系统生产蛋白质
相关领域
生物
病毒学
细胞生物学
病毒进入
病毒复制
轮状病毒
基因组
抄写（语言学）
呼肠孤病毒科
RNA沉默
体外
计算生物学
核糖核酸
遗传学
病毒
基因
RNA干扰
语言学
哲学
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| 网址 | |
| DOI | |
| 其它 | Baculovirus expression systems are well established as an easy and reliable way to produce high quality recombinant proteins. Baculoviruses can also be used to transduce mammalian cells, termed ‘BacMam’, with considerable potential in biomedical applications. This chapter explains the process of making a recombinant baculovirus, encompassing production of a recombinant virus by homologous recombination in insect cells, followed by amplification and titration of the virus—all steps needed before commencing gene expression and protein production. We also cover the use of small-scale test expression to provide an initial indication of quality and protein yield. Whereas proteins expressed at high levels can be directly scaled up, more challenging proteins may require optimization of cell lines, growth conditions, or harvest times. |
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