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Production of Chymosin using recombinant DNA technology
利用重组DNA技术生产凝乳酶
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| 其它 | AbstrcateThe milk clotting enzymes could be produced from different sources including microorganisms, plants, animals and recombinant rennet. Among these sources, the animal rennet especially bovine has a high to proteolytic activity ratio which leads good flavors textures in cheese. Due production limitations rising cost of rennet, past decades numerous attempts have been made substitute with other proteases or its using biotechnological approaches. Recent advances molecular biology DNA technology provide suitable, efficient effective approach for chymosin. In years cDNA gene encoding chymosin cloned characterized such as calf, camel, goat lamb successfully expressed hosts E. coli, Bacillus species, Aspergillus yeast plants (tobacco). So that now constitute 80% global chymosin’s market. host exhibit also cheese similar non-recombinant Furthermore, expression can improved through use advanced genetics protein engineering tools. |
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