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奥兰诺芬
程序性细胞死亡
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癌症研究
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预酸化
类风湿性关节炎
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其它 | MDCK, U87 and U251 cells were seeded at a density of 1 x 10' cells per well in 96 well plates at37 °C under normoxia and hypoxia conditions. After 24 h, the cell media was aspirated, 100 uL of500 ng mL-' of FLuc mRNA encapsulated ATP-) LNPS,ATP(+) LNPs and 1500 uM ATPdissolved in cell media were added into cells, followed by incubation for 24 h. Finally. the ATpconcentration was measured using luminescent ATp detection assay kit followed themanufacturer's protocol using a SpectraMax microplate reader with luminescence function. |
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