敬老院1号 管理员Lv12 关闭了本次求助。

说明 求助违规（查看求助规则）：该文献不存在补充材料(官网不存在或未提供)【积分已退回】

mealies Lv9 进行了留言

@mealies 因为输入字符有限，所以删了很多，你可以自己去下载看看，应该是你需要的。

他的参考文献里（https://doi.org/10.1016/S0009-9120(02)00283-7 ）确实有这个

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mealies Lv9 进行了留言

@mealies 同学，你这个文献指定没有这个补充材料，他是引用的21参考文献，我刚才看了一下，他文章的参考文献21中确实有描述，建议你去看他的参考文献的内容，如下：2.2. DNA sequence analysis
Genomic DNA was prepared from the patient’s whole blood (Puregene, Gentra System, Minneapolis, MN). The polymerase chain reaction (PCR) method was used to amplify the entire coding region of the LPL gene, using previously described amplification primers [22]. Samples were amplified for 30 cycles in a DNA Thermocycler (Perkin Elmer). Each cycle consisted of a denaturing step at 94°C for 30 s, annealing at 43 to 58°C for 30 s, and extension at 72°C for 30 s. Every reaction tube contained 32 pmol of each primer, 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1.5 mM MgCl2, 50 mM K

因为输入字符有限，所以删了很多，你可以自己去下载看看，应该是你需要的。

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mealies Lv9 进行了留言

@研友_pnx7JL DNA Analysis部分描述DNA was extracted as described.21 Coding regions and intron-exon boundaries of LPL (10 exons), APOC2 (4 exons), and APOA5 (4 exons) were amplified, purified, and then directly sequenced in 5-
and 3- directions in an ABI 3730 DNA Analyzer (Applied Biosystems) using reagents shown in supplemental Table I.

同学，你这个文献指定没有这个补充材料，他是引用的21参考文献，我刚才看了一下，他文章的参考文献21中确实有描述，建议你去看他的参考文献的内容，如下：2.2. DNA sequence analysis
Genomic DNA was prepared from the patient’s whole blood (Puregene, Gentra System, Minneapolis, MN). The polymerase chain reaction (PCR) method was used to amplify the entire coding region of the LPL gene, using previously described amplification primers [22]. Samples were amplified for 30 cycles in a DNA Thermocycler (Perkin Elmer). Each cycle consisted of a denaturing step at 94°C for 30 s, annealing at 43 to 58°C for 30 s, and extension at 72°C for 30 s. Every reaction tube contained 32 pmol of each primer, 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1.5 mM MgCl2, 50 mM K

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研友_pnx7JL 求助人 Lv2 进行了留言

@mealies 从上到下找了3遍，都没找到补充材料，你确定有，或者表达清楚一点。

DNA Analysis部分描述DNA was extracted as described.21 Coding regions and intron-exon boundaries of LPL (10 exons), APOC2 (4 exons), and APOA5 (4 exons) were amplified, purified, and then directly sequenced in 5-
and 3- directions in an ABI 3730 DNA Analyzer (Applied Biosystems) using reagents shown in supplemental Table I.

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研友_pnx7JL 求助人 Lv2 驳回了 jiqixi 上传的文件

说明

正文，需要补充材料

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mealies Lv9 进行了留言

从上到下找了3遍，都没找到补充材料，你确定有，或者表达清楚一点。

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jiqixi Lv1 上传了文件

已驳回 ATVBAHA.107.150680.pdf (98.05 KB) 回复

研友_pnx7JL 求助人 Lv2 发起了本次求助