化学
CLs上限
体内
隐形眼镜
甲基丙烯酰胺
银纳米粒子
生物利用度
角膜炎
纳米颗粒
细胞毒性
生物物理学
抗菌剂
纳米技术
组合化学
体外
核化学
共聚物
药理学
材料科学
生物化学
有机化学
聚合物
医学
生物技术
生物
验光服务
皮肤病科
丙烯酰胺
眼科
作者
Li Ma,Kaijun Li,Jiali Xia,Chaojian Chen,Yuqi Liu,Shiying Lang,Ling Yu,Haibo Wang
标识
DOI:10.1016/j.jcis.2021.11.145
摘要
The introduction of various drugs onto commercial soft contact lenses (CLs) has emerged as a potentially effective strategy for treating microbial keratitis (MK) because drug-loaded CLs can maintain a controlled drug concentration which leaded to enhanced drug bioavailability and reduced side effects in ocular tissues. In this study, silver nanoparticles modified with zwitterionic poly (carboxybetaine-co-dopamine methacrylamide) copolymer (PCBDA@AgNPs) as novel anti-infective therapeutics were prepared and firmly immobilized onto soft CLs through mussel-inspired surface chemistry. The obtained PCBDA@AgNPs coated CL (PCBDA@AgNPs-CL) remained the excellent transparency of commercial CLs and exhibited strong and broad-spectrum antimicrobial activities. We systematically explored the mechanism and found that the functional CLs can effectively inhibit the growth of microbial biofilms via a synergic "resist-kill-remove" strategy due to the zwitterionic surface and sustained release of silver ions. Significantly, in vitro cell cytotoxicity and in vivo subcutaneous implantation experiments proved the significant biosafety of PCBDA@AgNPs-CL. Furthermore, PCBDA@AgNPs-CL was successfully employed for the in vivo treatment of MK rabbit models, demonstrating excellent abilities to eradicate microbe-induced ocular infections and to prevent the destruction and irreversible structural alterations of corneal tissues. Collectively, PCBDA@AgNPs-CL is therefore a highly promising therapeutic device to significantly boost the efficacy for MK treatment.
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