花瓣
同源异型基因
生物
牵牛花
MADS箱
恶毒的
形态发生
花被
细胞生物学
分生组织
表皮(动物学)
雌蕊
转录因子
植物
遗传学
突变体
解剖
拟南芥
基因
花粉
雄蕊
作者
Mathilde Chopy,Quentin Cavallini-Speisser,Pierre Chambrier,Patrice Morel,Jérémy Just,Véronique Hugouvieux,Suzanne Rodrigues Bento,Chloé Zubieta,Michiel Vandenbussche,Marie Monniaux
标识
DOI:10.1093/plcell/koad258
摘要
Floral homeotic MADS-box transcription factors ensure the correct morphogenesis of floral organs, which are organized in different cell layers deriving from distinct meristematic layers. How cells from these distinct layers acquire their respective identities and coordinate their growth to ensure normal floral organ morphogenesis is unresolved. Here, we studied petunia (Petunia x hybrida) petals that form a limb and tube through congenital fusion. We identified petunia mutants (periclinal chimeras) expressing the B-class MADS-box gene DEFICIENS in the petal epidermis or in the petal mesophyll, called wico and star, respectively. Strikingly, wico flowers form a strongly reduced tube while their limbs are almost normal, while star flowers form a normal tube but greatly reduced and unpigmented limbs, showing that petunia petal morphogenesis is highly modular. These mutants highlight the layer-specific roles of PhDEF during petal development. We explored the link between PhDEF and petal pigmentation, a well-characterized limb epidermal trait. The anthocyanin biosynthesis pathway was strongly down-regulated in star petals, including its major regulator ANTHOCYANIN2 (AN2). We established that PhDEF directly binds to the AN2 terminator in vitro and in vivo, suggesting that PhDEF might regulate AN2 expression and therefore petal epidermis pigmentation. Altogether, we show that cell layerspecific homeotic activity in petunia petals differently impacts tube and limb development, revealing the relative importance of the different cell layers in the modular architecture of petunia petals.
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