生物分析
病危
加药
抗生素
化学
医学
重症监护医学
色谱法
药理学
生物化学
作者
John Takyi-Williams,Abbie D. Leino,Ruiting Li,Kevin J. Downes,Athena F. Zuppa,Amanda Bwint,Bo Wen,Duxin Sun,Marc H. Scheetz,Manjunath P. Pai
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2024-01-01
卷期号:16 (1): 19-31
标识
DOI:10.4155/bio-2023-0171
摘要
Background: Volumetric absorptive microsamples (VAMS) can support pharmacokinetic / pharmacodynamic studies. We present the bioanalytical method development for the simultaneous quantification of ampicillin, cefepime, ceftriaxone, meropenem, piperacillin, tazobactam, and vancomycin from VAMS. Methods & results: Optimal extraction, chromatographic, and mass spectrometry conditions were identified. Maximum extraction recoveries included 100 μl of water for rehydration and methanol for protein precipitation. Chromatographic separation used Phenomenex Kinetex™ Polar C18 column with a mobile phase comprising water/acetonitrile with formic acid and was fully validated. Hematocrit effects were only observed for vancomycin. Samples were stable for 90 days at -80°C except for meropenem, which was stable for 60 days. Conclusion: Multiple antibiotics can be assayed from a single VAMS sample to facilitate pharmacokinetic/pharmacodynamic studies.
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