排序酶A
肽聚糖
金黄色葡萄球菌
分拣酶
化学
脂质Ⅱ
荧光素
细菌
劈理(地质)
微生物学
部分
生物化学
生物物理学
荧光
细胞壁
立体化学
生物
古生物学
物理
量子力学
断裂(地质)
遗传学
作者
Jiang Feng,Chengteng Cai,Xiumin Wang,Shoufa Han
标识
DOI:10.1016/j.bmcl.2023.129428
摘要
Imaging or killing of a specific pathogen is of significance for precise therapy. Staphylococcus aureus (S. aureus) is an infectious gram-positive bacteria relying on Sortase A (SrtA) to anchor cell surface protein on peptidoglycan. We herein report signal-on labeling of S. aureus with self-quenched optical probes featuring vancomycin-conjugated SrtA substrate that is flanked by a dabcyl moiety paired with either fluorescein or eosine photosensizer (PS). SrtA-mediated cleavage of the substrate motif releases the dabcyl quencher, leading to covalent labeling of peptidoglycan with fluorescein or PS of restored photophysical property. The dual biomarked-enabled peptidoglycan labeling enables signal-on imaging and effective photodynamic destruction of S. aureus, suggesting a protheranostic approch activatable to SrtA-positive bacteria engaged in myriad diseases.
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