CCR2型
5-羟色胺能
炎症
创伤性脑损伤
TLR4型
医学
内科学
神经炎症
血清素
内分泌学
免疫学
受体
趋化因子
趋化因子受体
精神科
作者
Mahmoud G. El Baassiri,Zachariah Raouf,Hee‐Seong Jang,Daniel Scheese,Johannes W. Duess,William B. Fulton,Chhinder P. Sodhi,David J. Hackam,Isam Nasr
出处
期刊:The journal of trauma and acute care surgery
[Ovid Technologies (Wolters Kluwer)]
日期:2024-01-08
标识
DOI:10.1097/ta.0000000000004246
摘要
Abstract Background Traumatic brain injury (TBI) leads to acute gastrointestinal dysfunction and mucosal damage, resulting in feeding intolerance. Ccr2 + monocytes are crucial immune cells that regulate the gut’s inflammatory response via the brain-gut axis. Using CCR2 KO mice, we investigated the intricate interplay between these cells to better elucidate the role of systemic inflammation after TBI. Methods A murine-controlled cortical impact model was utilized, and results were analyzed on post-injury days (PID) 1 and 3. The experimental groups included (1) Sham C57Bl/6 wild-type (WT), (2) TBI WT, (3) Sham CCR2 KO and (4) TBI CCR2 KO . Mice were euthanized on PID 1 and 3 to harvest the ileum and study intestinal dysfunction and serotonergic signaling using a combination of quantitative real-time PCR (qRT-PCR), immunohistochemistry, FITC-dextran motility assays, and flow cytometry. Student’s t-test and one-way ANOVA were used for statistical analysis, with significance achieved when p < 0.05. Results TBI resulted in severe dysfunction and dysmotility of the small intestine in WT mice as established by significant upregulation of inflammatory cytokines iNOS, Lcn2, TNFα, and IL1β and the innate immunity receptor toll-like receptor 4 (Tlr4). This was accompanied by disruption of genes related to serotonin synthesis and degradation. Notably, CCR2 KO mice subjected to TBI showed substantial improvements in intestinal pathology. TBI CCR2 KO groups demonstrated reduced expression of inflammatory mediators (iNOS, Lcn2, IL1β, and Tlr4) and improvement in serotonin synthesis genes, including tryptophan hydroxylase 1 (Tph1) and dopa decarboxylase (Ddc). Conclusion Our study reveals a critical role for Ccr2 + monocytes in modulating intestinal homeostasis after TBI. Ccr2 + monocytes aggravate intestinal inflammation and alter gut-derived serotonergic signaling. Therefore, targeting Ccr2 + monocyte-dependent responses could provide a better understanding of TBI-induced gut inflammation. Further studies are required to elucidate the impact of these changes on brain neuroinflammation and cognitive outcomes. Study type Original Article (Basic Science, level of evidence N/A)
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