幽门螺杆菌
抗生素
微生物学
抗生素耐药性
化学
生物
药理学
遗传学
作者
Junyuan Xue,Wen Li,Yican Zhao,Liyuan Wang,Peng Cheng,Lu Zhang,Yingkui Zheng,Wenxin Zhang,Y. J. Bi,Zhenghong Chen,Tuo Jiang,Yuan Sun
摘要
ABSTRACT The increasing antibiotic resistance of Helicobacter pylori primarily driven by genetic mutations poses a significant clinical challenge. Although previous research has suggested that antibiotics could induce genetic mutations in H. pylori , the molecular mechanisms regulating the antibiotic induction remain unclear. In this study, we applied various techniques (e.g., fluorescence microscopy, flow cytometry, and multifunctional microplate reader) to discover that three different types of antibiotics could induce the intracellular generation of reactive oxygen species (ROS) in H. pylori . It is well known that ROS, a critical factor contributing to bacterial drug resistance, not only induces damage to bacterial genomic DNA but also inhibits the expression of genes associated with DNA damage repair, thereby increasing the mutation rate of bacterial genes and leading to drug resistance. However, further research is needed to explore the molecular mechanisms underlying the ROS inhibition of the expression of DNA damage repair-related genes in H. pylori . In this work, we validated that ROS could trigger an allosteric change in the iron uptake regulatory protein Fur, causing its transition from apo-Fur to holo-Fur, repressing the expression of the regulatory protein ArsR, ultimately causing the down-regulation of key DNA damage repair genes (e.g., mutS and mutY ); this cascade increased the genomic DNA mutation rate in H. pylori . This study unveils a novel mechanism of antibiotic-induced resistance in H. pylori , providing crucial insights for the prevention and control of antibiotic resistance in H. pylori .
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