材料科学
PLGA公司
小泡
子宫内膜异位症
纳米颗粒
膜
细菌外膜
腹膜
重编程
细胞生物学
生物医学工程
纳米技术
病理
医学
生物
细胞
生物化学
基因
大肠杆菌
作者
Ning Wu,Zheyi Han,Wenxing Lv,Yanjuan Huang,Jingwen Zhu,Jinqi Deng,Qing Xue
出处
期刊:Biomaterials
[Elsevier BV]
日期:2025-02-17
卷期号:319: 123198-123198
被引量:15
标识
DOI:10.1016/j.biomaterials.2025.123198
摘要
Endometriosis is a chronic inflammatory disease that primarily affects women of reproductive age. The current hormonal treatments are unsuitable for women who wish to conceive, highlighting the need for non-hormonal therapeutic alternatives. In this study, we engineered outer membrane vesicle (OMV)-coated poly (lactic-co-glycolic acid) (PLGA) nanoparticles (OMV-NPs) as a potential therapy for endometriosis. These OMV-NPs were internalized by macrophages more efficiently than bacterial OMVs and preserved the immunostimulatory properties of OMVs. In vivo administration of OMV-NPs in mice achieved prolonged retention in the peritoneal cavity, with effective uptake by nearly 80 % of the peritoneal macrophages. Notably, treatment with OMV-NPs reprogrammed macrophages toward the M1 phenotype, resulting in a significant decrease in the M2 to M1 ratio within the peritoneal cavity and in endometriotic lesions. This shift from M2 to M1 was associated with reduced TGF-β1 production and suppressed myofibroblast activation, which led to substantial inhibition of endometriosis progression. Furthermore, immunohistochemical imaging of paired eutopic and ectopic endometrial tissues from endometriosis patients revealed a positive correlation between M2-polarized macrophages and fibrosis. This finding suggests that reprogramming macrophages with OMV-NPs could be a promising therapeutic approach for endometriosis.
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