抗菌剂
生物制药
壳聚糖
微生物学
抗菌肽
肽
沙门氏菌
胃肠道
细菌
生物膜
肠衣
药理学
化学
生物
生物化学
生物技术
剂型
遗传学
作者
Cesar Augusto Roque‐Borda,Mauro de Mesquita Souza Saraiva,Wagner Dias Macedo,José Carlos Estanislao Márquez Montesinos,Andréía Bagliotti Meneguin,Anna Beatriz Toledo Borges,Edson Crusca,Saulo Santesso Garrido,A. Bugalho de Almeida,Reinaldo Marchetto,Marlus Chorilli,Ângelo Berchieri,Silvio Rainho Teixeira,Fernando Rogério Pavan,Eduardo Festozo Vicente
出处
期刊:Biomaterials
[Elsevier]
日期:2023-02-01
卷期号:293: 121978-121978
被引量:7
标识
DOI:10.1016/j.biomaterials.2022.121978
摘要
The incorrect use of conventional drugs for both prevention and control of intestinal infections has contributed to a significant spread of bacterial resistance. In this way, studies that promote their replacement are a priority. In the last decade, the use of antimicrobial peptides (AMP), especially Ctx(Ile21)-Ha AMP, has gained strength, demonstrating efficient antimicrobial activity (AA) against pathogens, including multidrug-resistant bacteria. However, gastrointestinal degradation does not allow its direct oral application. In this research, double-coating systems using alginate microparticles loaded with Ctx(Ile21)-Ha peptide were designed, and in vitro release assays simulating the gastrointestinal tract were evaluated. Also, the AA against Salmonella spp. and Escherichia coli was examined. The results showed the physicochemical stability of Ctx(Ile21)-Ha peptide in the system and its potent antimicrobial activity. In addition, the combination of HPMCAS and chitosan as a gastric protection system can be promising for peptide carriers or other low pH-sensitive molecules, adequately released in the intestine. In conclusion, the coated systems employed in this study can improve the formulation of new foods or biopharmaceutical products for specific application against intestinal pathogens in animal production or, possibly, in the near future, in human health.
科研通智能强力驱动
Strongly Powered by AbleSci AI