细菌
致病菌
化学
硝基还原酶
斑马鱼
病菌
荧光
芸苔属
生物标志物
微生物学
计算生物学
生物化学
生物
植物
酶
遗传学
基因
物理
量子力学
作者
Yi‐Xuan Fu,Wu‐Yingzheng Guo,Nan Wang,Yi‐Jie Dai,Zi-Ye Zhang,Xinlin Sun,Wen‐Chao Yang,Guang‐Fu Yang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-12-05
卷期号:94 (50): 17692-17699
被引量:11
标识
DOI:10.1021/acs.analchem.2c04614
摘要
Plant diseases caused by bacteria have become one of the serious problems that threaten human food security, which led to the remarkable reduction of agricultural yields and economic loss. Nitroreductase (NTR), as an important biomarker, is highly expressed in bacteria, and the level of NTR is closely related to the progression of pathogen infection. Therefore, the design of small-molecule fluorescent sensors targeting NTR is of great significance for the detection and diagnosis of plant pathogenic bacteria. In this study, a new fluorescent sensor targeting NTR was discovered and then successfully applied to the imaging of zebrafish and pathogenic bacteria. Most importantly, the developed sensor achieved the real-time diagnosis of Brassica napus L. infected with bacteria, which provides a promising tool for examining the temporal and spatial infection of plant pathogens in precision agriculture.
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