中国仓鼠卵巢细胞
传染性
细胞培养
逆转录病毒
内源性逆转录病毒
内生
维罗细胞
逆转录酶
病毒学
生物
分子生物学
病毒
转染
细胞
A549电池
仓鼠
HEK 293细胞
前病毒
中国仓鼠
细胞生物学
化学
RNA定向DNA聚合酶
体外
合胞体
伽马逆转录病毒
类病毒颗粒
绿色荧光蛋白
作者
Nicholas Mattson,Trent J. Bosma,Yamei Gao,Sandra M. Fuentes,Pei-Ju Chin,Arifa S. Khan
出处
期刊:Viruses
[Multidisciplinary Digital Publishing Institute]
日期:2025-10-23
卷期号:17 (11): 1408-1408
摘要
The Chinese hamster ovary K1 cell line (CHO-K1) constitutively produces retroviral-like particles (RVLPs) containing reverse transcriptase (RT) activity, which, thus far, have not been shown to be infectious. Since infectious retroviruses have been reported in other rodent species, this study was undertaken to investigate the presence of latent, infectious, endogenous retroviruses (ERVs) in CHO-K1 cells by using chemical induction assays and detection of activated virus using the highly sensitive, product-enhanced RT (PERT) assay, with subsequent infectivity analysis in cell lines of different species, including human. The results demonstrated activation of A-type and C-type retroviral particles based on transmission electron microscopy and increased production of cell-free RT-particles after treatment of the cells with 5-iodo-2′-deoxyuridine and 5-azacytidine, which was greater with dual treatment than with each inducer alone. Induction of A- and C-type particles was confirmed in dual-drug-treated CHO-K1 cells by long-read high-throughput sequence (HTS) analysis. Infectivity studies performed by inoculating human A549, HEK-293, and MRC-5 cells; African green monkey Vero cells; Mus dunni cells; and CHO-K1 cells with supernatant containing RT-particles from dual-treated CHO-K1 cells indicated the absence of a replicating retrovirus in supernatant from extended cell culture using the PERT assay. Furthermore, short-read HTS analysis did not show evidence of integration of retroviral sequences in inoculated A549 and 293 cells. The overall results showed no evidence for latent, infectious, endogenous RVLPs in CHO-K1 cells.
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