A Pump-Free SERS Microfluidic Chip Based on Identification-Competition Strategy for Ultrasensitive and Efficient Simultaneous Detection of Liver Cancer-Related microRNAs

In this study, we present a pump-free SERS microfluidic chip capable of detecting liver cancer-related miR-21 and miR-155 concurrently with ultra-sensitivity and high efficiency. We employed a Fe 3 O 4 @cDNA-AuNPs@Raman reporter@H composite structure and a recognition competition strategy. When the target miRNAs (miR-21 and miR-155) are present in the test liquid, they specifically compete with the nucleic acid complementary strand(H) of Fe 3 O 4 @cDNA-AuNPs@Raman reporter@H, causing AuNPs to competitively detach from the surface of Fe 3 O 4 , resulting in a decrease in the SERS signal. Consequently, this pump-free SERS microfluidic chip enables the detection of the target miRNAs more rapidly and accurately in complex environments. This method offers an approach for the simultaneous and efficient detection of miRNAs and holds promising applications in the early diagnosis of liver cancer.