互补DNA
基础(拓扑)
信使核糖核酸
碱基对
核糖核酸
化学
胸苷
生物
计算生物学
分子生物学
生物化学
DNA
基因
数学
数学分析
作者
David Sturgill,Daniel Arango,Shalini Oberdoerffer
出处
期刊:STAR protocols
[Elsevier]
日期:2022-12-01
卷期号:3 (4): 101858-101858
标识
DOI:10.1016/j.xpro.2022.101858
摘要
N4-acetylcytidine (ac4C) is an mRNA modification catalyzed by the enzyme N-acetyltransferase 10 (NAT10), with position-dependent effects on mRNA translation. This protocol details a procedure to map ac4C at base resolution using NaBH4-induced reduction of ac4C and conversion to thymidine followed by sequencing (RedaC:T-seq). Total RNA is ribodepleted and then treated with NaBH4 to reduce ac4C to tetrahydro-ac4C, which specifically alters base pairing during cDNA synthesis, allowing the detection of ac4C at positions called as thymidine following Illumina sequencing. For complete details on the use and execution of this protocol, please refer to Arango et al. (2022).1.
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