3D DNA walker recognition-driven homogeneous dual-mode sensing strategy based on enzyme biofuel cell for ultrasensitive detection of HER2

An ultrasensitive enzyme biofuel cell (EBFC) based self-powered biosensor is developed for electrochemical/colorimetric dual-mode detection of human epidermal growth factor receptor 2 (HER2). Graphdiyne (GDY) and tetragonal molybdenum disulfide (1 T MoS 2 ) are prepared to construct high-performance EBFC. Three-dimensional (3D) DNA Walker triggers hybrid chain reaction (HCR) cascade strategy is used to further improve the output signal intensity and selectivity of assay. Furthermore, a capacitor is matched to EBFC to generate electrical signals magnified several times for boosting the analytical performance. An ultrasensitive dual-mode assay is therefore developed for HER2 with an expanded linear response range of 0.1-10000 pg mL -1 and a low detection limit of 0.03 pg/mL ( S/N =3). The results confirm the developed assay provides a powerful tool for ultrasensitive and accurate detection of cancer marker. • An ultrasensitive self-powered biosensor is developed for dual-mode detection of HER2. • Graphdiyne and 1 T metal phase molybdenum disulfide are prepared to construct high-performance EBFC. • DNA walker triggers hybrid chain reaction cascade strategy further greatly improve the sensitivity. • Capacitor is used to amplify output signal for boosting analytical performance.