半胱氨酸
石墨烯
DNA
荧光
材料科学
硫醇
逻辑门
转身(生物化学)
猝灭(荧光)
氧化物
纳米技术
组合化学
化学
生物化学
计算机科学
物理
算法
酶
冶金
量子力学
作者
Youhui Lin,Yu Tao,Fang Pu,Jinsong Ren,Xiaogang Qu
标识
DOI:10.1002/adfm.201101584
摘要
Abstract In this work, a unique, highly sensitive and selective fluorescence turn‐on approach for cysteine detection using an ensemble of graphene oxide (GO) and metallized DNA is reported. The method is based on the extraordinarily high quenching efficiency of GO and the specific interaction between cysteine and metallized DNA via robust Ag–S bonds. In the presence of GO, the dye‐labeled single‐stranded DNA shows weak fluorescence, while it exhibits a dramatic fluorescence increase upon the formation of the double helix through the “activated” metallized DNA by cysteine. In addition, the protocol shows excellent selectivity for cysteine over various other amino acids found in proteins. Importantly, by exploring GO–DNA interactions and the thiol‐mediated DNA hybridization, our sensing system can also be utilized to design the “OR” and “INHIBIT” logic gates using cysteine and DNA as inputs. To the author's knowledge, this method is the first example of combining GO and DNA metallization to fabricate a turn‐on fluorescent sensor for cysteine and logic gates.
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